[HTML][HTML] Phosphorylation of cold shock domain/Y-box proteins by ERK2 and GSK3β and repression of the human VEGF promoter
LS Coles, L Lambrusco, J Burrows, J Hunter… - FEBS letters, 2005 - Elsevier
FEBS letters, 2005•Elsevier
The hypoxia responsive region (HRR) of the VEGF promoter plays a key role in regulating
VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA
and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB
are phosphorylated by ERK2 and GSK3β in vitro, and the binding of dbpB to single-strand
VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the
ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating …
VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA
and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB
are phosphorylated by ERK2 and GSK3β in vitro, and the binding of dbpB to single-strand
VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the
ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating …
The hypoxia responsive region (HRR) of the VEGF promoter plays a key role in regulating VEGF expression. We found that the cold shock domain (Y-box) repressor proteins, dbpA and dbpB/YB-1, bind distinct strands of the human VEGF HRR. We find both dbpA and dbpB are phosphorylated by ERK2 and GSK3β in vitro, and the binding of dbpB to single-strand VEGF HRR DNA is regulated by this phosphorylation. These findings suggest the ERK/MAPK and PI3K pathways may regulate VEGF expression in part through regulating the action of these repressor proteins.
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