Failure to process dentin matrix protein 1 (DMP1) into fragments leads to its loss of function in osteogenesis
Dentin matrix protein 1 (DMP1), an acidic protein important to the formation of bone and
dentin, primarily exists as the processed NH 2-terminal and COOH-terminal fragments in the
extracellular matrix of the two tissues. Previous in vitro studies showed that the substitution
of residue Asp 213 by Ala 213 (D213A) at a cleavage site blocked the processing of mouse
DMP1 in cells. In this study, we generated transgenic mice expressing mutant D213A-DMP1
(WT/D213A-Tg mice) to test the hypothesis that the proteolytic processing of DMP1 is an …
dentin, primarily exists as the processed NH 2-terminal and COOH-terminal fragments in the
extracellular matrix of the two tissues. Previous in vitro studies showed that the substitution
of residue Asp 213 by Ala 213 (D213A) at a cleavage site blocked the processing of mouse
DMP1 in cells. In this study, we generated transgenic mice expressing mutant D213A-DMP1
(WT/D213A-Tg mice) to test the hypothesis that the proteolytic processing of DMP1 is an …
