Three forms of macromolecular translocation inhibitor of the nuclear binding of “activated” receptor-glucocorticoid complex and their interaction with ATP
K Okamoto, F Isohashi, M Horiuchi… - … and biophysical research …, 1982 - Elsevier
K Okamoto, F Isohashi, M Horiuchi, Y Sakamoto
Biochemical and biophysical research communications, 1982•ElsevierThree macromolecular translocation inhibitors of nuclear translocation or binding of
“activated” receptor-[3 H] triamcinolone acetonide in rat liver cytosol were eluted from a
DEAE-cellulose column with a linear gradient of NaCl. The macromolecular material, eluted
first from the column with about 0.1 M NaCl (Peak I), had the inhibitory activity, but in the
presence of 5 mM ATP this fraction enhanced the nuclear binding. The inhibitory effect of the
second peak (Peak II), eluted with about 0.2 M NaCl, was abolished by the addition of ATP …
“activated” receptor-[3 H] triamcinolone acetonide in rat liver cytosol were eluted from a
DEAE-cellulose column with a linear gradient of NaCl. The macromolecular material, eluted
first from the column with about 0.1 M NaCl (Peak I), had the inhibitory activity, but in the
presence of 5 mM ATP this fraction enhanced the nuclear binding. The inhibitory effect of the
second peak (Peak II), eluted with about 0.2 M NaCl, was abolished by the addition of ATP …
Three macromolecular translocation inhibitors of nuclear translocation or binding of “activated” receptor-[3H]triamcinolone acetonide in rat liver cytosol were eluted from a DEAE-cellulose column with a linear gradient of NaCl. The macromolecular material, eluted first from the column with about 0.1 M NaCl (Peak I), had the inhibitory activity, but in the presence of 5 mM ATP this fraction enhanced the nuclear binding. The inhibitory effect of the second peak (Peak II), eluted with about 0.2 M NaCl, was abolished by the addition of ATP. The inhibitor in Peak III, eluted with about 0.3 M NaCl, was not influenced by ATP. These results suggest that there are at least three inhibitors in rat liver cytosol, and that they are distinct entities, judging from their positions of elution on chromatography and the effects of ATP on their actions.
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